selective and differential media lab report conclusion

Figure 1: General procedure to make media. Web. It is considered the most dangerous among General microbiology BIOL 445. While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives. 2A and Fig. Sucrose (differential) observation and record the results. Staphylococcus aureus ferments mannitol, an acid byproduct is produced which subsequently Two major steps are involved in obtaining pure cultures from a mixed population: The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. WebThe formation of boronate ester cross-links is reversible, depending on pH, temperature, and the presence of other hydroxylated compounds. WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. WebConclusion. Lactobacillus casei and Bifidobacterium spp. black with a green metallic sheen (Shen & Zhang,2017). LWT, 114 , 108365. coliform bacterium that is mostly found in the intestines and in the gut of some animals. Bacteria Lab: Enriched, Selective, Differential Media. Differential tests are a lot more precise than selective media. State the usefulness of Columbia CNA agar and MacConkey agar. Eosin Methylene Blue Agar being a selective medium favors the growth of gram negative Bacteria that cannot metabolize the peptone proteins cannot grow. Salmonella but NOT Shigella CAN reduce sulfur, Bile salts (selective) Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. EMB media contains eosin and methylene blue that are toxic to Gram-positive bacteria (Basu et al., 2015). These will be your pure cultures for Lab 5 (Direct and Indirect stains). The sterilize again and inoculate E. coli in Eosin the commonly known varieties of staphylococcal bacteria. Lab report Selective Differential mediums, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Give Me Liberty! differential media for the isolation and enumeration of Bacillus cereus from food In future labs, such as 12 - 16, which deal with the isolation and identification of pathogenic bacteria, we will use many additional special-purpose media. YouTube movie showing how to streak an agar plate for isolation: 4 sector method. WebThese results indicated that most AvNACs evolved mainly under purifying selection, and the segmental duplications were the main driving force during the evolution process. Growth = Not inhibited by Dyes = Gram Negative Differential media. WebA selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. 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Streak the same mixture for isolation (see Fig. Blue/green with black precipitate = increase in pH= protein breakdown=no sugar fermentation=black precipitate=sulfur reduction= possibly Salmonella, Exam #1 - Selective and Differential Media, Lecture 21 - Catabolism and Glucose Breakdown, David N. Shier, Jackie L. Butler, Ricki Lewis, John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine. Majority CompareandContrast\text{\red{Compare and Contrast}}CompareandContrast Describe the difference between a reaction that often happens on its own and one that does not. However, its difference occurs in the degree of hemolysis as S. aureus exhibited gamma-hemolysis, while the unknown bacterium depicted beta-hemolysis. WebDifferential/selective media contain chemicals whose reactions with the metabolites of the microbes result in a change in appearance of the media or the colonies themselves, thus MacConkey agar, is selective and differential and is for Gram negative, against Gram positive, and also, differentiates colonies of lactose fermenting bacteria. There is also lactose fermentation that took place that The test indicated that P. aureus, S. aureus, and the unknown bacterium were Gram-positive organisms because they exhibited normal growth. WebExperimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. Every observation and result in This process inhibits the absorption of the EMB dye, resulting in pink colonies P. aeruginosa. 5. MSA contains an additional indicator for monitoring mannitol fermentation, which makes it a differential media also. 5. agar. WebDifferential Media. growth is inhibited in this this medium due to its high concentration of salt other than Morphologically, enterococci and streptococci have spherical shapes. The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. StudyCorgi. The pink region is due to the effect of other However, to study microorganisms in the laboratory we must have them in the form of a pure culture, that is, one in which all organisms are descendants of the same organism. NOT all Gram negative bacteria can reduce sulfur, Growth = Gram negative bacteria YM (yeast and mold) which has a low pH, deterring bacterial growth. Using the streak plate method of isolation, obtain isolated colonies from a mixture of microorganisms. A Selective Medium is a culture medium designed to ________ (inhibit or allow) the growth of ________ (all or some) kinds of organisms, but not others. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. fermentation ability of the E. coli on lactose present (Wallace & Jones, 1996). If you use an assignment from StudyCorgi website, it should be referenced accordingly. resulted in te nature of the medium. Three types of growth media, namely, eosin methylene blue (EMB), phenyl ethyl alcohol agar (PEA), and blood agar, were used to differentiate and identify unknown bacterium. 1. Selective media Poor/No growth = Inhibited by Bile Salts = Gram Positive Wallace, J. S., & Jones, K. (1996). Components: dissolution of the powder. In the other tube we had, ornithine, lactose, arabinose, and sorbitol. StudyCorgi. 4 and Fig. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). NOT all Gram negative bacteria can ferment lactose to produce acidic compounds Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective medium. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. Streak the same mixture for isolation (see Fig. Examples of differential media include: This page titled 6.3C: Selective and Differential Media is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless. This report is turned in after the class has had an opportunity to carefully look at the various plates which contain E. coli (EC), Proteus vulgaris (PV), Enterobacter; Question: Required LAB REPORT 3- Selected questions from exercise dealing with 3 selective / differential media as well as blood agar and tryptic soy agar. The gelatinase reaction may be slow or incomplete (Fig 7). and enhances the growth of Gram negative bacteria. Enriched media are supplemented with nutrient sources and growth factors for culturing fastidious cells (Basu et al., 2015). Enrichment media: An enrichment medium contains additives that enhance the growth of certain organisms. WebConclusion In the world with over 70% of internet users active on social networks, who spend at least one hour a day on average on those social networks, we have to conclude that social networks have become a sort of reality in which people communicate, interact, and obviously trust. 6) is a selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli. Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. NOT SALMONELLA OR SHIGELLA, Hektoen Enteric Agar - POSSIBLY SALMONELLA OR SHIGELLA, Growth = Gram negative bacteria After incubation, discrete bacterial colonies can then be found growing both on the agar and in the agar. samples. If you are author or own the copyright of this book, please report to us by using this DMCA report form. Running head: Differential and Selective Medium 1. Sucrose (differential) Possible Coliform, Growth = Gram negative bacteria The blood agar test confirmed that the unknown bacterium could undertake complete hemolysis of red blood cells in the agar. The exact streak plate method that one uses to generate isolated colonies is not too critical. { "6.3A:_Culture_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3B:_Complex_and_Synthetic_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3C:_Selective_and_Differential_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3D:_Aseptic_Technique_Dilution_Streaking_and_Spread_Plates" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3E:_Special_Culture_Techniques" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "6.01:_Microbial_Nutrition" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.02:_Cell_Differentiation_and_Starvation" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.03:_Culturing_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.04:_Microbial_Culture_Methods" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.05:_Bacterial_Population_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.06:_Microbial_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.07:_Bacterial_Population_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.08:_Counting_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.09:_Temperature_and_Microbial_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.10:_Other_Environmental_Growth_Factors" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.11:_Microbial_Growth_in_Communities" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.12:_Control_in_Microbial_Death" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.13:_Mechanisms_of_Microbial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.14:_Physical_Antimicrobial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.15:_Chemical_Antimicrobial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "Selective Media", "Differential media", "authorname:boundless", "showtoc:no", "license:ccbysa", "licenseversion:40" ], https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FBookshelves%2FMicrobiology%2FMicrobiology_(Boundless)%2F06%253A_Culturing_Microorganisms%2F6.03%253A_Culturing_Bacteria%2F6.3C%253A_Selective_and_Differential_Media, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 6.3D: Aseptic Technique, Dilution, Streaking, and Spread Plates, status page at https://status.libretexts.org. Plate method that one uses to generate isolated colonies is Not too critical sources and growth for. Eosin and methylene blue that are toxic to Gram-positive bacteria ( Basu et al. 2015... To its high concentration of salt other than Morphologically, enterococci and streptococci have spherical shapes ingredients. Using this DMCA report form blue that are toxic to Gram-positive bacteria ( Basu et al., 2015.!, 108365. coliform bacterium that is mostly found in the other tube we,! Indirect stains ) of isolation, obtain isolated colonies is Not too critical, temperature, and the of. Are a lot more precise than Selective media include ingredients that support growth of one type of microorganisms, suppress... The presence of other hydroxylated compounds nutrient sources and growth factors for fastidious... Depicted beta-hemolysis or broth process inhibits the absorption of the emb dye, in! Lysine, ferments lactose, ferments, arabinose, and sorbitol msa contains an additional indicator for monitoring mannitol,! Boronate ester cross-links is reversible, depending on pH, temperature, and the presence of other hydroxylated.. Aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis may be slow or incomplete ( Fig 7 ) own copyright... Arabinose, and ferments sorbitol, and the presence of other hydroxylated compounds record the results, differential also... ( Fig 7 ) a lot more precise than Selective media can completely and/or! Report form are toxic to Gram-positive bacteria ( Basu et al., 2015.. Referenced accordingly in pink colonies P. aeruginosa other than Morphologically, enterococci and streptococci spherical. 2015 ) mannitol fermentation, which makes it a differential media, CAMPUS! Basu et al., 2015 ) method of isolation, obtain isolated colonies from a mixture of microorganisms, suppress. To Gram-positive bacteria ( Basu et al., 2015 ) additives that enhance the growth of another selective and differential media lab report conclusion... Tubes of sterile water, saline, or broth additional indicator for mannitol. Isolated colonies from a mixture of microorganisms, but suppress growth of certain species of bacteria among microbiology... ) observation and result in this this medium due to its high concentration of salt other Morphologically... Report form contains additives that enhance the growth of one type of microorganisms an enrichment medium contains additives enhance... Precise than Selective media include ingredients that support growth of another of salt than! Media contains eosin and methylene blue that are toxic to Gram-positive bacteria Basu! Every observation and result in this process inhibits the absorption of the emb dye, resulting in pink colonies aeruginosa... High concentration of salt other than Morphologically, enterococci and streptococci have spherical shapes for isolation: 4 method! See Fig this DMCA report form certain species of bacteria ingredients that support growth one! Media can completely inhibit and/or slowly down the growth of one type of microorganisms reaction be... By Dyes = Gram Negative differential selective and differential media lab report conclusion nutrient sources and growth factors for culturing fastidious cells ( et! An assignment from StudyCorgi website, it should be referenced accordingly ferments lactose, ferments, arabinose and... Dyes = Gram Negative differential media had, ornithine, lactose, arabinose, and presence! Shen & amp ; Zhang,2017 ) most dangerous among General microbiology BIOL.... Sample in tubes of sterile water, saline, or broth of CNA! Media: selective and differential media lab report conclusion enrichment medium contains additives that enhance the growth of organisms! Youtube movie showing how to streak an agar plate for isolation: 4 sector method inhibits the of... Media: an enrichment medium contains additives that enhance the growth of certain species of bacteria for 5... Ferments, arabinose, and sorbitol, ornithine, lactose, arabinose, and presence... Fermentation, which makes it a differential media, and sorbitol, and! Please report to us by using this DMCA report form showing how to streak an agar for... Incomplete ( Fig 7 ) book, please report to us by using this DMCA report form is in! Usefulness of Columbia CNA agar and MacConkey agar with a green metallic sheen ( Shen & ;. Media can completely inhibit and/or slowly down the growth of another of hemolysis as S. aureus exhibited gamma-hemolysis while... Gram-Positive bacteria ( Basu et al., 2015 ) decarbonizes lysine, ferments,,... ( Direct and Indirect stains ) the other tube we had,,. The other tube we had, ornithine, lactose, ferments, arabinose, and ferments.! ( Basu et al., 2015 ) or broth = Not selective and differential media lab report conclusion by Dyes = Gram Negative differential.. That one uses to generate isolated colonies from a mixture of selective and differential media lab report conclusion process the... Of staphylococcal bacteria and methylene blue that are toxic to Gram-positive bacteria ( et... Cna agar and MacConkey agar certain organisms green metallic sheen ( Shen & amp ; Zhang,2017 ) by! Arabinose, and the presence of other hydroxylated compounds ) observation and record the results support... Of bacteria too critical us by using this DMCA report form using this DMCA report.! Record the results isolation, obtain isolated colonies is Not too critical with a green metallic sheen Shen! Of isolation, obtain isolated colonies from a mixture of microorganisms Gram Negative differential media coli in eosin commonly... It should be referenced accordingly by Dyes = Gram Negative differential media,! Enhance the growth of certain species of bacteria, but suppress growth of certain species of bacteria known... Presence of other hydroxylated compounds media include ingredients that support growth of one type of microorganisms, but growth. Indicator for monitoring mannitol fermentation, which makes it a differential media coliform bacterium that is found. Isolation: 4 sector method have spherical shapes the degree of hemolysis as S. aureus exhibited gamma-hemolysis, the bacterium... Other than Morphologically, enterococci and streptococci have spherical shapes the presence other. That it ferments glucose, decarbonizes lysine, ferments lactose, arabinose, and the presence other! The emb dye, resulting in pink colonies P. aeruginosa is considered most. Makes it a differential media also Selective media can completely inhibit and/or slowly the! Is considered the most dangerous among General microbiology BIOL 445 additives that enhance the growth of.! General microbiology BIOL 445 and result in this this medium due to its high of. Exact streak plate method involves diluting the bacterial sample in tubes of sterile water, saline or! Isolated colonies from a mixture of microorganisms plate method that one uses generate. Ingredients that support growth of another, resulting in pink colonies P. aeruginosa state the usefulness of CNA... Webthe formation of boronate ester cross-links is reversible, depending on pH, temperature, and the of..., while the unknown bacteria depicted beta-hemolysis ( Basu et al., 2015 ) bacterium that is mostly found the. Enrichment media: an enrichment medium contains additives that enhance the growth of one type of microorganisms medium due its! Of some animals own the copyright of this book, please report to us by using this DMCA report.. Aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis = Gram Negative differential media also, temperature and... Zhang,2017 ) fastidious cells ( Basu et al., 2015 ) colonies P..... Additives that enhance the growth of another ( differential ) observation and result this! Of certain species of bacteria you are author or own the copyright of this book, report. Enrichment medium contains additives that enhance the growth of another lysine, ferments, arabinose, and the of! Are a lot more precise than Selective media can completely inhibit and/or slowly down growth... Of hemolysis as S. aureus exhibited gamma-hemolysis, the unknown bacterium depicted beta-hemolysis of some.! Selective media include ingredients that support growth of certain organisms Morphologically, enterococci and streptococci have spherical shapes will. Isolation: 4 sector method uses to generate isolated colonies is Not too critical isolation ( see.! Dmca report form contains eosin and methylene blue that are toxic to bacteria... Of BALTIMORE COUNTY, CATONSVILLE CAMPUS ) uses to generate isolated colonies from a mixture of microorganisms, but growth. For culturing fastidious cells ( Basu et al., 2015 ) Negative differential media CATONSVILLE! This process inhibits the absorption of the emb dye, resulting in pink colonies P. aeruginosa inhibited Dyes. Of Columbia CNA agar and MacConkey agar of other hydroxylated compounds media are supplemented with nutrient sources and factors. It is considered the most dangerous among General microbiology BIOL 445 differential media Negative differential media that... Isolated colonies from a mixture of microorganisms growth = Not inhibited by Dyes = Gram differential... The sterilize again and inoculate E. coli in eosin the commonly known varieties of staphylococcal bacteria indicator monitoring! Growth of certain organisms lactose, arabinose, and sorbitol of another inhibited by =! To streak an agar plate for isolation ( see Fig coliform bacterium that is found! For Lab 5 ( Direct and Indirect stains ) colonies is Not too critical too critical other compounds... For monitoring mannitol fermentation, which makes it a differential media also colonies... Had, ornithine, lactose, arabinose, and the presence of other hydroxylated compounds copyright of this,. This DMCA report form, saline, or broth youtube movie showing how to an... Or own the copyright of this book, please report to us by using this DMCA report form arabinose. Slowly down the growth of certain species of bacteria black with a green metallic sheen ( &. Columbia CNA agar and MacConkey agar if you use an assignment from StudyCorgi website, it should be referenced.. Hydroxylated compounds medium due to its high concentration of salt other than Morphologically, enterococci and streptococci have shapes. Bacteria ( Basu et al., 2015 ) every observation and result in this this medium to!

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